Catalase Lab

Lab Report Catalase Enzyme Lab Catalase Enzyme Lab Background: Catalse is an enzyme unremarkably found in more plants and animals. Its purpose is to defend glossary off any toxic substances by destroying them. It is also engage by some cells to destroy cellular waste. This lab lead demonstrate the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be hydrogen hydrogen bleach (H2O2). Catalase kit and boodle by the following mechanism. 2H2O2 ( 2H2O + O2 What is the effect of temperature and pH on the action of catalase? Aims: Demonstrate and observe the effect of surface field of honour of the enzyme, temperature and pH on the military action of catalase Materials: 1M. NaOHHot Plate10 ml graduated cylinder 1M. HClIcedistilled water 3% H2O22 full-size beakerspotato 6 test resistance systemsForcepsliver Test tobacco pipe rack3 micropipettesmortar and pestle 3 thermomete rknifeStop arrest Test subway holderStirring rod Part A conventionality Catalase Reaction guessing: modest potato will adopt the highest rate of reaction part: 1- Take two comely test subways and mark them 1 and 2. 2- Place a switch of potato in render 1. 3- effuse 2ml of hydrogen bleach to tube 2, and determine temperature. 4- Pour content of tube 2 to tube 1, and observe. 5- Pour off the liquid in tube 1, leaving the piece of potato. 6- contribute another 2 ml of hydrogen peroxide to tube 1, and observe.

7- Using the mortar and pestle, thump out a piece of potato and place it in a clean test tube (3). 8- Pour 2ml of hydrogen peroxide into tu! be 3, and observe. Data accrual: Quantitative Table 1: Recyclability and surface area effects on enzymatic activity |Type of reaction |Uncrushed potato (control) (22 c) |Recycled potato (catalase) |Crushed white potato | |Rate of reaction |3 |1...If you want to rile a full essay, order it on our website: OrderEssay.net

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